Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence.

نویسندگان

  • Olivier Dussurget
  • Emilie Dumas
  • Cristel Archambaud
  • Ingrid Chafsey
  • Christophe Chambon
  • Michel Hébraud
  • Pascale Cossart
چکیده

In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. We also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of L. monocytogenes. A comparative proteomic analysis revealed an effect of the fri deletion on the levels of listeriolysin O and several stress proteins. Together, our study demonstrates that fri has multiple roles that contribute to Listeria virulence.

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عنوان ژورنال:
  • FEMS microbiology letters

دوره 250 2  شماره 

صفحات  -

تاریخ انتشار 2005